Education.. Employment.. Research Interests.. Publications..

• Education︰

  • 1994-1999 Ph. D., Department of Crystallography, University of Pittsburgh, U. S. A.
  • 1989-1991 M. S., Institute of Life Sciences, National Tsing-Hua University, Taiwan
  • 1985-1989 B.S., Physics Department, National Tsing-Hua University, Taiwan

• Employment︰

  • 2002-present Joint Associate Professor, Physics Dept., National Tsing-Hua University
  • 2001-present Associate Research Scientist, NSRRC
  • 2001-2002 Part-time Assistant Professor, Physics Dept., National Tsing-Hua University
  • 1999-2000 Postdoc., Dept. of Biochemistry & Molecular Biology, Univ. of Georgia, U. S. A.

  　

• Research Interests︰

  1. Synchrotron X-ray Protein Crystallography
  2. Structural Biology & Structural Genomics
  3. Biophysics
  4. Biochemistry & Molecular Biology

  　

• Publications︰
1. Journal Papers:
   1. Chen, C.-J., Liu, Z.-J., Rose, J. P., and Wang, B.-C. (1999) “Low-salt crystallization of T7 RNA polymerase: a step towards the transcription bubble complex.” Acta Crystallographica, D55, 1188-1192.
   2. Schubot, F. D, Chen, C.-J., Rose, J. P. and Wang, B.-C. (2000) “Crystallization and preliminary X-ray diffraction analysis of the mitochondrial transcription factor sc-mtTFB from Saccharomyces cerevisiae.” Acta Cryst., D56, 902-903.
   3. Liu, Z.-J., Vysotski, E. S., Chen, C.-J., Rose, J. P., Lee, J. and Wang, B.-C. (2000) “Structure of the Ca+2-regulated photoprotein obelin at 1.7 Å resolution determined directly from its sulfur substructure.” Protein Science, 11, 2085-2093. (equal contribution as the first author)
   4. Chen, C.-J., Rose, J. P., Rosenbaum, G. and Wang, B.-C. (2000) “Low-resolution sulfur ‘super atom’ SAD phasing of macromolecules containing disulfide bonds.” American Crystallographic Association Newsletter, 3, 28.
   5. Schubot, F. D., Chen, C.-J., Rose J. P., Dailey, T. A., Dailey, H. A., Wang, B.-C. (2001) Crystal structure of the transcription factor sc-mtTFB offers insights into mitochondrial transcription. Protein Science, 10, 1980-1988.
   6. Chen, C.-J.* and Chiang, C.-M. (2002) “Crystallization and preliminary crystallographic study of transglutaminase from Streptomyces”. Acta Crystallographic, A58, C90.
   7. Liaw, S.-H., Chen, S.-J., Ko, T.-P., Hsu, C.-S., Chen, C.-J., Wang, A. H.-J., and Tsai, Y.-C. (2003) “Crystal structure of D-aminoacylase from Alcaligenes faecalis DA1. A novel subset of amidohydrolases and insights into the enzyme mechanism”. J. Biol. Chem. 278, 4957-4962.
   8. Chen, C.-J.*, Liu, M.-Y., Chang, C., Payne, W. J., Wang, B.-C., and LeGall, J. (2003) “Crystal structure of nucleoside diphosphate kinase from Bacillus halodenitrificans at 2.2 Å resolution, an Enzyme Co-expressed of its Activity with a Mn-superoxide Dismutase”. J. Struct. Biol., 142, 247-255.
   9. Huang, J.-Y., Chang, C.-Y., Chang, T., and Chen, C.-J.* (2003) “Crystallization and preliminary X-ray crystallographic analysis of nucleoside diphosphate kinase from rice” Acta Cryst., D59, 1648-1650.
   10. Chen, C.-J.*, Liu, M.-Y., Chen, Y.-T., and LeGall, J. (2003) “Preparation and X-ray crystallography analysis of rubredoxin crystals from Desulfovibrio gigas to beyond ultra-high 0.68 Å resolution” Biochem. Biophys. Res. Comm., 308, 684-688.
   11. Wang, Y.-L., Goh, K.-X., Wu, W-g., and Chen, C.-J.* (2004) “Purification, crystallization and preliminary X-ray crystallographic analysis of a cysteine-rich secretory protein (CRISP) from Naja atra venom”, Acta Cryst., D60, 1912-1915.
   12. Huang, J.-Y, Chang, T, Chen, C.-J.* (2004) “Crystal Structure of nucleoside diphosphate kinase from rice”, NSRRC 2003/2004 Activity Report (Highlight Article), p.p. 75-77.
   13. Lee, S.-C., Guan, H.-H., Wang, C.-H., Huang, W.-N., Chen, C.-J.*, and Wu, W.* (2004) “Structural basis of venom citrate-dependent heparan sulfate-mediated cell surface retention of cobra cardiotoxin A3”. J. Biol. Chem. Dec 6, in Press ahead of publication. 10.1074/jbc.M412398200
   14. Chen, C.-Y., Ko, T.-P., Chou, C.-C., Lin, T.-W., Chen, C.-J., Wang, A. H.-J.* (2005) “Probing the DNA kink structure induced by the hyperthermophilic chromosomal protein Sac7d”, Nucleic Acids Research, 33, 430-438.
   15. Huang, J.-Y, Chang, T, Chang, C.-Y., and Chen, C.-J.* (2005) “Crystal structure of a nucleoside diphosphate kinase required for coleoptile elongation in rice (Oryza sativa L.)”, J. Struct. Biol. In press. (JSB-04-99)
   16. Hsieh, Y.-C., Liu, M.-Y., Le Gall, J. and Chen, C.-J.* (2005) “Anaerobic purification and crystallization to improve the crystal quality: ferredoxin II from Desulfovibrio Gigas” Acta Cryst. D61, In press (IC5039)
   17. Hsieh, Y.-C., Liu, M.-Y., and Chen, C.-J.* (2005) “Anaerobic and Aerobic Structures of Ferredoxin II from Desulfovibrio Gigas”, SPring-8 Research Frontiers. In press.
   18. Wang, Y.-L., Lee, S.-C., Goh, K.-S., Huang, W.-N., Wu, W.-g.*, and Chen, C.-J.* (2005) “Sulfur SAD crystal structure of CRISP from Naja atra: 3D structure of a heparin binding snake venom CRISP reveals distinct protease and ion channel blocking domain for its function”, J. Biol. Chem. Revised. (M4:12358)
   19. Chen, T.-S., Chung, F.-Y., Chang, S.-C., Huang, W.-N., Chien, K.-Y., Wu, P.-L., Goh, K.-S., Lin, H.-C., Chen, C.-J.*, Wu, W.* (2005) “Group I cobra cardiotoxins as conformational analogues of cyclolinopeptide A”, Biochemistry. Revised. (BI050172E)
   20. Huang, Y.-C, Chang, T., Shih, C.-L., Shih, C.-H., Lin, Y.-H., and Chen, C.-J.* (2005) “Purification, crystallization and preliminary X-ray crystallographic analysis of rice lectin from Oryza sativa”, Acta Cryst. D. Submitted.
   21. Chen, C.-J.*, Liu, M.-Y., Chen, Y.-T., and LeGall, J. (2005) “Crystal structure of rubredoxin from Desulfovibrio gigas using Fe-SAD method and direct method at ultra-high 0.68 Å resolution”, in preparation.
   22. Hsieh, Y.-C., Liu, M.-Y., Le Gall, J., and Chen, C.-J.* (2005) “A comparison of aerobic and anaerobic crystal structures of ferredoxin II from Desulfovibrio gigas using Fe-SAD method at ultra-high 0.9 Å resolution”, in preparation.
   23. Shih, C.-H., Huang, Y.-J, Lin, Y.-H. Chang, T., and Chen, C.-J.* (2005) “Crystal structure of rice lectin from Oryza sativa L.”, in preparation.
   24. Kuo, C.-Y., Tsai, H.-J., Pan, Y.-H., Li, Y.-K., and Chen, C.-J.* (2005) “Mechanistic action and crystal structure of chitinase from Bacillus cereus NCTU2 at 1.2 Å resolution” J. Bacteriol. In preparation.

2. Books：
   1. Chen, C.-J., Rose, J. P., Liu, Z.-J., Newton, G., and Wang, B.-C. (2002)“Protein Crystallography”, in Practical Methods in Advanced Protein Chemistry/Catalog Number 9453; Brown, W. E. and Howard, G. C., Ed. CRC Press, Boca Raton. p.p. 7-36.

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